BGE® HTP Antibody Expression
In order to meet the needs of customers for screening a large number of antibody samples in a short time in the process of antibody drug development, DetaiBio launched BGE® HTP Antibody Expression service, which can express antibodies in small quantities and quickly, can be delivered as soon as 2 weeks. Self built high-throughput recombinant antibody expression platform, the throughput can reach 1000 single / month. The service is based on mammalian cell expression system, combined with self-developed proEM™ high expression vector was transfected into CHO/HEK293 cells for recombinant antibody expression. The final delivery of recombinant antibody with purity >90%, and the requirements of HPLC purity, endotoxin and concentration can be customized according to your downstream application to solve your worries.
Service Features
High Throughput
HTP process from gene synthesis to antibody purification
Highly Efficient
Two-week fastest
delivery
Flexible Choice
Flexible choice of
expression scale
Various forms
Full-length Antibody、Fab、scFv、VHH、Chimeric Antibody and etc.
Procedure
Workflow
| Stage | Service | Timeline |
|---|
| Stage I: Gene Synthesis (optional) | - Codon Optimization
- Gene Synthesis
| 1 week |
| Stage II: Vector Construction | - Vector construction
- Sequencing validation
- Plasmid extraction
|
| Stage III: Antibody Expression & Purification | - Cell Transient transfection (1~20mL)
- Antibody Expression & Purification
| 1 week |
| Stage IV: QC & Deliverables | - Purified Antibody
- CoA
- Cloning Plasmid
| 3 days |
|
Case Study
Full-length Antibody
Expression&Purification
Lane M: SDS-PAGE Protein
Lane M: Marker
Lane 1: Cell Culture Supernatant
Lane 2: Effluent
Lane 3-5: Elution Component
SEC-HPLC purity>99%Related Service
