Product Description
The His-tag consists of six histidines (His-His-His-His-His-His), typically attached to a protein's N- or C-terminus, facilitating recombinant protein purification. Due to its small molecular weight, it generally does not affect the protein's structure or bioactivity, making it one of the most widely used purification tags.
Detai Bio offers a Rapid His tag protein detection kit, utilizing lateral flow immunoassay technology for qualitative detection in cell culture supernatants, cell/bacterial lysates, or purified His-tag proteins. Simply add the sample to the test strip and read results within 10 minutes.
Product Advantages
- Speed: Results in 10 minutes
- Accuracy: Comparable to other antibody immunoassays
- Ease of Use: No dilution needed for supernatants and lysates, no additional equipment required
- Reproducibility: Consistent color and results across batches
Application Scenarios
- Verify His-tagged protein expression
- Optimize protein induction time during expression
- Check elution fractions during protein purification
Product Overview
| Product Name | His tag protein detection kit |
| Purpose | Qualitative detection of His-tagged proteins in various samples |
| Technology | Lateral flow immunoassay |
| Storage | 4-30°C, avoid direct sunlight, do not freeze |
| Validity | 12 months |
Product Contents (10 tests)
| Rapid His-tag Protein Test Strips | 10 tests |
| Sample Dilution Buffer | 10 mL |
Instructions for Use
1. Sample Requirements
①Applicable for cell culture supernatant, cell/bacterial lysates, or purified protein products. If cloudy, centrifuge and use the supernatant.
②No dilution needed for supernatants or lysates; purified samples can be diluted if needed.
③Samples must be at room temperature before testing.
2. Test Method
①Allow the test strip to reach room temperature.
②Insert the strip into the sample or add 75 μL sample to the strip’s sample pad, avoiding bubbles. Read results after 10 minutes.
3.Results Interpretation
①Negative: Red bands appear on control (C) and test (T) lines.
②Positive: Red band on C line only, no band on T line.
③Invalid: No red band on C line.
Q: What is the optimal sample concentration for the His-tag Protein Rapid Detection Kit?
A: ≥2 µg/mL.
Q: Do I need controls when using the His-tag Protein Rapid Detection Kit?
A: Yes, negative and positive controls must be set up for accurate results. It is recommended that the control samples and the test samples be of the same matrix.
Q: Why does the color intensity of the test line (T) vary after testing different samples?
A: When the sample contains no His-tagged protein, the test line (T) shows the darkest color. As the His-tagged protein content increases, the color intensity of the test line (T) gradually weakens until it disappears.
Q: Can the His-tag Protein Rapid Detection Kit be used for quantitative detection?
A: No. This kit provides qualitative detection of His-tagged protein. For quantification, use relevant specialized instruments.
