Prokaryotic (E. coli) System Expression Service
E. coli expression system is the most widely used and economical protein expression system at present. E. Coli has many advantages including clear genetic background, convenient operation, fast proliferation, low cost, high expression volume and good stability. It is suitable for the expression of a variety of generic proteins, especially for the production of small molecular proteins, but prokaryotic expression is easy to form insoluble inclusion bodies. DetaiBio focused on protein expression for more than ten years with rich experience in inclusion body renaturation, and can provide one-stop service from codon optimization to recombinant protein expression and purification.
Service Features
Highly Experienced
- 3000+ successfully delivered recombinant protein ;
- Rich experience in inclusion body renaturation
High Success Rate
- Success rate > 95%;
- Multiple packages
- "no protein, no charge "
Independent R&D
- Improved soluble expression of protein
- Optimization of culture conditions
One-stop Service
- One-stop service from codon optimization to recombinant protein expression and purification
Workflow
Sequence Analysis & Codon Optimization
1 day
- Customer provides target protein sequence.
- DetaiBio analyzes and optimizes rare codons, codon preferences and other factors.
Gene Synthesis & Vector Construction
1 week
- Synthetic gene Sequencing validation.
- Cloned into the proprietary high-efficiency expression vector of DetaiBio.
Expression Test & Solubility Analysis
1 week
- Different strains were selected to optimize various expression conditions to improve protein soluble expression.
Scale-up Expression & Purification
1-2 weeks
- Select appropriate purification methods and combine with advanced AKTA purification equipment to purify protein
Deliverables: 0.5mg protein; CoA; Cloning plasmid 1μg；Gene sequencing report；Service workflow report
Case Study
Case 1: Expression and Purification of Unlabeled Protein in E. coli
①Electrophoretic test results of supernatant after centrifugation of bacteria
Lane M：Protein marker
Lane 1~2：Supernatant after centrifugation of bacteria
②Protein electrophoresis results after primary purification
Lane M：Protein marker
Lane 1：Protein after PEI and hydrophobic column
③Protein electrophoresis results after secondary purification
Lane M：Protein marker
Lane 1~10：Sephracyl S-100 purified protein
Case 2: Renaturation and Purification of Inclusion Body
①Purification results of primary affinity chromatography after renaturation
Lane M: SDS-PAGE Protein Marker
Lane 1: Supernatant after inclusion body dissolution
Lane 2:Supernatant after incubation with Ni-IDA
Lane 3-6: 50 mM Imidazole elution component
Lane 7-12: 500 mM Imidazole elution component
②Purification results of secondary ion exchange chromatography
Lane M: SDS-PAGE Protein Marker
Lane 1: Effluent after Ni-IDA purification
Lane 2: Effluent after incubation of SP beans 6ff column
Lane 3-4: 50 mM NaCl elution component
Lane 5-8: 500 mM NaCl elution component
Related Services
Related Resources
