Product Description
Detaibio Flag-tagged protein rapid detection kit uses lateral chromatography immunoassay technology to quickly and qualitatively detect cell culture supernatants, cell/bacterial lysates or purified Flag-tagged proteins. When testing, you only need to add the test strip to the sample as indicated by the arrow, or add a small amount of sample to the test strip and wait for 10 minutes to read the test results.
This product is for scientific research use only.
Product Advantages
- Speed: Results in 10 minutes
- Accuracy: Comparable to other antibody immunoassays
- Ease of Use: No dilution needed for supernatants and lysates, no additional equipment required
- Reproducibility: Consistent color and results across batches
Application Scenarios
- Verify Flag-tagged protein expression
- Optimize protein induction time during expression
- Check elution fractions during protein purification
Product Overview
| Product Name | Flag tag protein detection kit |
| Purpose | Qualitative detection of Flag-tagged proteins in various samples |
| Technology | Lateral flow immunoassay |
| Storage | 4-30°C, avoid direct sunlight, do not freeze |
| Validity | 12 months |
Product Contents (10 tests)
| Rapid Flag-tag Protein Test Strips | 10 tests |
| Sample Dilution Buffer | 10 mL |
Instructions for Use
1. Sample Requirements
①Applicable for cell culture supernatant, cell/bacterial lysates, or purified protein products. If cloudy, centrifuge and use the supernatant.
②No dilution needed for supernatants or lysates; purified samples can be diluted if needed.
③Samples must be at room temperature before testing.
2. Test Method
①Allow the test strip to reach room temperature.
②Insert the strip into the sample or add 75 μL sample to the strip’s sample pad, avoiding bubbles. Read results after 10 minutes.
3.Results Interpretation
①Negative: Red bands appear on control (C) and test (T) lines.
②Positive: Red band on C line only, no band on T line.
③Invalid: No red band on C line.
Q: What is the optimal sample concentration for the Flag-tag Protein Rapid Detection Kit?
A: ≥1 µg/mL.
Q: Do I need controls when using the Flag-tag Protein Rapid Detection Kit?
A: Yes, negative and positive controls must be set up for accurate results. It is recommended that the control samples and the test samples be of the same matrix.
Q: Why does the color intensity of the test line (T) vary after testing different samples?
A: When the sample contains no Flag-tagged protein, the test line (T) shows the darkest color. As the Flag-tagged protein content increases, the color intensity of the test line (T) gradually weakens until it disappears.
Q: Can the Flag-tag Protein Rapid Detection Kit be used for quantitative detection?
A: No. This kit provides qualitative detection of Flag-tagged protein. For quantification, use a relevant quantitative assay kit.
