Product Description
Detaibio’s mouse IgG antibody subtype identification kit uses lateral chromatography immunoassay technology to quickly detect cell culture supernatant, ascites or purified mouse IgG antibody product subtypes. When testing, you only need to draw a small amount of sample and add it to the sample addition area of the test paper card, wait for 10 minutes, and then the test results can be read.
This product is for scientific research use only.
Product Overview
| Product Name | Rapid Mouse mAb IgG Isotyping Kit |
| Purpose | Detection of cell culture supernatant, ascites fluid or purified mouse IgG antibody product subtypes |
| Technology | Lateral flow immunoassay |
| Sensitivity | 20 ng/mL |
| Storage | 4-30°C, avoid direct sunlight, do not freeze |
| Validity | 12 months |
Product Contents (10 tests)
| Rapid Mouse mAb IgG Isotyping Test Strips | 10 tests |
| Sample Dilution Buffer | 15 mL |
Sample Requirement
①This reagent is suitable for detecting mouse IgG antibody products in cell culture supernatants, ascitic fluid, or purified antibodies. If the sample is visibly turbid, centrifuge and use the supernatant for testing
②Before testing, refrigerated samples should be brought to room temperature, and frozen samples should be fully thawed, mixed well, and then brought to room temperature before testing.
Test Method
①Sample dilution: (Please dilute according to the following ratios based on sample type)
1)Cell culture supernatant: 1:50 (sample: diluent), i.e., add 2 μL sample to 100 μL diluent.
2)Ascitic fluid: 1:1000 (sample: diluent), i.e., add 2 μL sample to 2 mL diluent.
3)Purified antibody: Dilute to 500 ng/mL using diluent.
4)If the antibody concentration in the sample is below 1 μg/mL, no dilution is needed; the sample can be added directly for testing.
*The above dilution methods are recommended for first-time testing. Adjustments can be made based on the sample concentration.
②Remove the test strip and place it flat on the surface, allowing it to equilibrate to room temperature.
③Take 80 μL of the sample and add it to the sample area on the reagent card, ensuring no visible air bubbles. Start the timer and read the result after 10 minutes.
Test Results
①Negative result: A red band appears at the control line (C line), and no red bands appear at the test lines (T1/T2/T3/T4/T5).
②Positive result: A red band appears at the control line (C line), and one or more red bands appear at the test lines (T1/T2/T3/T4/T5).
③Invalid result: No red band appears at the control line (C line). Retest the sample.
Q: What are the advantages of using an immunochromatographic strip for mouse IgG antibody subclass identification?
A: Currently, the determination of monoclonal antibody subclasses mainly relies on ELISA, which takes 4-6 hours per test, requiring significant time and effort. This severely delays the selection and preservation of monoclonal antibody purification methods, increasing the time and workload in the monoclonal antibody preparation process. This product is a rapid method for identifying the subclass of mouse monoclonal antibodies in cell culture supernatants or purified monoclonal antibodies using an immunochromatographic strip. It is convenient, quick, cost-effective, has high accuracy, good repeatability, and short detection time, solving the issues associated with ELISA testing.
Q: What are the sample requirements before using the mouse IgG antibody subclass identification kit?
A: Before testing, refrigerated samples should be brought to room temperature. Frozen samples must be fully thawed, mixed well, and brought to room temperature before testing. If the sample is visibly turbid, centrifuge and use the supernatant for testing.
Q: Why do the test line T color intensities vary after testing different samples?
A: When the sample does not contain His-tagged proteins, the test line T shows the darkest color. As the content of His-tagged protein in the sample increases, the intensity of the test line T color gradually weakens, eventually disappearing.
Q: What is the sample dilution ratio when using the mouse IgG antibody subclass identification kit?
A: Sample dilution: (Please dilute according to the following ratios based on sample type)
① Cell culture supernatant: 1:50 (sample : diluent);
② Ascitic fluid: 1:1000 (sample : diluent);
③ Purified antibody: Dilute to 500 ng/mL using diluent;
④ If the antibody concentration in the sample is below 1 μg/mL, no dilution is needed; use the sample directly for testing.
Ensure the dilution ratio follows the instructions for accurate results.
Q: What are the storage conditions and precautions when using the mouse IgG antibody subclass identification kit?
A: Store at 4-30°C, with a shelf life of 12 months. Avoid direct sunlight, store in a cool place, and do not freeze.
Q: Is a control group necessary when using the mouse IgG antibody subclass identification kit?
A: To ensure the accuracy of the results, it is recommended to set up both a negative and a positive control.
