Stable Cell Line Development
Different from transient transfection, stable cell line construction means that foreign genes enter the recipient cells and integrate into the chromosomes of the cells, so that the host cells can express the target genes for a long time. Stable cell lines have many applications, such as protein antibody production, signal transduction, drug target research, immunotherapeutic drug development, etc. the expressed protein / antibody has better stability and less batch difference.
DetaiBio provides mammalian stable cell line construction services, and the monoclonal screening process uses unique visual DeepLight® cell screening platform, which is 2-3 months faster than the traditional monoclonal screening process, shortens the development period of cell lines by over 70%.
Service Features
Faster Screening
One month: from DNA to cell line
Higher Screening Throughput
Screening 640K clones at one time
Visualized Screening Results
Optimized cell line with high expression
Stable and High Yield
Recombinant monoclonal antibody can reach 5 g/L
Advantages of DeepLight® cell screening platform？
| Limiting Dilution Analysis | DeepLight® Screening |
|---|
| Screening Period | 8 Weeks | One Day |
| Cell Separation Efficiency | Low | High |
| Screening Throughput | Low | High |
| Single Cell Screening | No | Yes |
Workflow
Gene Synthesis
1 week
- Codon optimization
- Gene synthesis
- Vector construction
- Plasmid Preparation
Cell Transfection
1 week
- Cell transfection
- Expression detection
- WB or ELISA
Stable Cell Line Screening
2-4 weeks
- On-chip screening
- MSX screening
- Stability evaluation
- Cell cryopreservation
Deliverables
- 2 selected stable cell lines
- CoA
- Sequening report
QC：SDS-PAGE、WB、SEC-HPLC (optional)、Other Tests (optional)
Case Study
DeepLight® Visualized Screening Results
Cell Line with Stable and High Yield
